Isothermal amplification is a molecular biology technique that allows for the rapid amplification of DNA at a constant temperature, typically around 60-65°C. This method is advantageous because it does not require the use of complex equipment, such as thermocyclers, which are needed for traditional PCR amplification. There are several methods of isothermal amplification, including loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), helicase-dependent amplification (HDA), and nucleic acid sequence-based amplification (NASBA). These techniques have been widely used in various applications, such as the detection of pathogens, genetic mutations, and forensic analysis. Isothermal amplification is particularly useful in resource-limited settings or field situations where access to sophisticated laboratory equipment is limited. It offers a cost-effective and efficient alternative to traditional PCR methods for nucleic acid amplification.